This 4-day practical course covers current theory and best practices with regard to derivation and culture.
Established in 2015 in collaboration with the UK Stem Cell Bank, this residential course includes lecture, discussion and practical laboratory sessions covering the following topics:
- Good Cell Culture Practice (GCCP) and aseptic processing
- Approaches for reprogramming of somatic cells (reprogramming methodologies – virus and episomal; somatic cell types – skin fibroblasts and PBMCs)
- Cryopreservation and recovery
- Passaging
- Quality control (identity, karyology, mycoplasma, sterility, cell markers)
- Pluripotency testing
Learning Outcomes
After attending this course, participants should be able to:
- Recognise key principles of GCCP and aseptic processing including the role of antibiotics
- Assess and identify key advantages, disadvantages and critical elements of different approaches to somatic cell reprogramming
- Assess and identify undifferentiated, fully reprogrammed iPSCs compared to partially reprogrammed iPSC colonies
- Recognise critical aspects for successful preservation and storage
- Evaluate the recovery of iPSCs and identify viable cells
- Identify undifferentiated morphology of confluent iPSCs ready for passaging
- Identify and troubleshoot critical aspects in the passaging and maintenance of undifferentiated iPSCs
- Recognise the importance of core QC and potential consequences of failing to carry it out
- Evaluate different capabilities of each method of determining potential pluripotency
Speakers: Lead Instructors: Professor Glyn Stacey UK Stem Cell Bank (The National Institute for Biological Standards and Control (NIBSC), UK), Dr Minal Patel (Wellcome Trust Sanger Institute, UK)
Category: Conferences | Science, Health and Medicine | Stem Cells
WebsiteVisit website
VenueWellcome Genome Campus
Address, Hinxton, Cambridgeshire,
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Nearest Hotels
Staying overnight in Hinxton?
We've teamed up with Booking.com to provide the best room rates for hotels near the vicinity of the Derivation And Culture Of Human event in Hinxton.
The Red Lion Hinxton
In a tiny village south of Cambridge, this appealing 16th-century freehouse has an AA Rosette restaurant, an atmospheric bar, and new-build en suite rooms with free internet..
The Crown House Hotel
This former Georgian coaching inn located in the picturesque village of Great Chesterford, just off the M11 motorway. It offers an award-winning restaurant, a huge landscaped garden and free parking..
Duxford Lodge Hotel
Overlooking landscaped gardens, with awards for hospitality and cuisine, this 3-star hotel has luxury bedrooms and a quality restaurant. Close to Cambridge, Newmarket and the Science Parks..
Holiday Inn Express Cambridge
The brand new Holiday Inn Express Cambridge Duxford M11 Jct 10 offers comfortable rooms with power showers and Wi-Fi access. Cambridge centre is a 20-minute drive away, and the hotel offers free parking..
The Red Lion Hotel
The Red Lion Hotel dates back to the 13th century and features wooden beams hand-carved by Carmelite monks. The inn is situated in Whittlesford Bridge, just 2 miles from Duxford, and offers elegant rooms with Hypnos beds..
Whittlesford Bed and Breakfast
Close to Duxford Airfield and the historic city of Cambridge, this homely B&B offers free Wi-Fi and free parking on site. It is situated in Whittlesford, just 5 minutes drive from the M11 motorway..
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